【摘要】 目的利用乳果糖（Lactulose，L）和甘露醇（Mannitol，M）测定肠粘膜通透性的方法，已被广泛接受和应用。目前尚无正常国人乳果糖和甘露醇排出比值的资料。本研究目的为测定正常国人口服乳果糖、甘露醇后尿中的排除率比值（L／M）。方法用带脉冲电化学检测器的高压液相仪（HPLC-PED）分别测定60例健康志愿者口服以往剂量（乳果糖10g、甘露醇5g）和小剂量（乳果糖2g、甘露醇1g）测试液后，尿中的乳果糖和甘露醇浓度。计算乳果糖和甘露醇排泄率及其比值。结果在以往剂量试验中，甘露醇和乳果糖6小时尿中的排出率分别为9．40±2．3％（6％～13．1％）和0．2％±0．10（0．10～0，4％）。L／M比值为0．026±0．006（0．019～0．036）。在小剂量试验中，甘露醇和乳果糖6小时尿中的排出率分别为21．0％±4．2％（15．7％～26．5％）和0．8％±0．30（0．40～1．20）。L／M比值为0．036±0．008（0．028～0．052）。甘露醇和乳果糖的CV值分别为1．61％和3．52％。甘露醇和乳果糖的回收率分别为101．12％±3％和98．4％±3％。结论应用HPLC-PED方法提高了检测灵敏度，可减少乳果糖和甘露醇的用量，降低了副作用的发生率，更利于临床应用。测量乳果糖、甘露醇排除率比值反映肠粘膜通透性，是临床实用的方法。更多还原

【Abstract】 Purpose To determine Lactulose (L) /Mannitol (M) excretion ratio with HPLC-PED in healthy Chinese volunteers. Methods 60 healthy Chinese volunteers enrolled. For each volunteers the L/M permeability study was performed twice by two different doses,small dose test (1g mannitol and 2g lactulose) and reported dose test (5g mannitol and 10g lactulose). The urine were collected during 6 hours after ingestion of the test solution. The M and L concentration were measured by HPLC with Pulsed Electrochermical Detection (HPLC-PED). Results The L/M ratio was 0. 026±0.006 in the Small dase test, and 0.036 ±0.008 in the reported dose test. Conclusion With HPLC-PED, low dose of lactulose and mannitol could be used to study the intestinal permeability. Determination of the L/M ratio might be a way of evaluating intestinal barrier function in clinical practice.更多还原