【摘要】 目的:研究丁基苯酞（NBP）对局灶性脑缺血再灌注引起的血脑屏障（BBB）破坏的保护作用。方法:尼龙线栓塞法造成大鼠局灶性脑缺血模型,尼龙线抽出进行再灌。BBB的通透性以结合有伊文氏蓝染料的血浆蛋白外渗进入脑组织的量以及免疫组化和电镜方法进行了测定。结果:脑缺血3h再灌注3h可使结合有血浆蛋白的伊文氏蓝在缺血皮层含量明显增加;免疫球蛋白IgG在大脑皮层中的表达明显增加;皮层组织中毛细血管内皮细胞膜上有微小孔洞的形成,内皮细胞中线粒体空泡化。NBP 5,10,和20mg·kg^-1可使皮层中染料含量分别下降33.3％,46.7％和50.7％;缺血皮层中IgG的表达减少;毛细血管超微结构的损伤也有一定程度的减轻。NBP20mg·kg^-1亦可使脑缺血再灌后脑水肿程度明显减轻。结论:NBP可减轻局灶性脑缺血再灌引起的BBB损伤。更多还原

【Abstract】 AIM: To study the protective effect of dl-3-n-butylphthalide (NBP) on blood-brain barrier (BBB) damage induced by reperfusion following focal cerebral ischemia. METHODS: Focal cerebral ischemia in rats was performed by inserting a nylon suture into intracranial segment of internal carotid artery to block the origin of middle cerebral artery and reperfusion by withdrawing the nylon suture. Permeability of BBB was determined by extravasation of the protein-bound Evans blue dye to cerebral cortex and further evaluated by immunohistochemical or electronmicroscopic method. RESULTS: Reperfusion for 3 h following focal cerebral ischemia for 3 h produced BBB damage which exhibited the increase in extravasation in cerebral cortex, elevation of the expression of immunoglobulin ( IgG), and pore formation in endothelial cell membrane of capillary in cerebral cortex. NBP (5 -20 mg·kg-1) decreased the extravasation in a dose-dependent manner. The expression of IgG in cerebral cortex was decreased and the ultrastructure damage of capillaries was alleviated after treatment with NBP. NBP 20 mg·kg-1 also alleviated brain edema caused by 3-h reperfusion following 3-h middle cerebral artery occlusion (MCAO). CONCLUSION: NBP hasprotective effect on BBB damage induced by reperfusion after MCAO.更多还原