【摘要】 目的:研究5-羟色胺释放剂芬氟拉明加强针刺镇痛前后大鼠脑内中央灰质(PAG)腹侧部单胺类递质的变化。方法:运用微透析及高效液相电化学检测方法。结果:电针后Nor的释放减少,而5-HT,5-HIAA和DA,HVA在AG部位含量升高(P<0.05,vs NS组)。当芬氟拉明合用电针时,5-HT,5-HIAA含量进一步升高,但Nor,DA及其代谢产物却无明显变化(P<0.05 vs NS+EA组)。结论:电针能促进DA和5-HT释放但抑制Nor释放。芬氟拉明合用电针能进一步加强5-HT的释放。芬氟拉明加强针刺镇痛可能与进一步激活5-羟色胺系统有关。更多还原

【Abstract】 AIM: To study the changes of monoamines in ventrolatoral periaqueductal gray of rat brain before and after electroacupuncture (EA) analgesia (EAA) was enhanced by fenfluramine (Fen), a 5-hydroxytrypta-mine (5-HT) releaser. METHODS: Monoamines were collected by in vivo microdialysis and measured by HPLC connected with electrochemical detector. RESULTS: The level of norepinephrine (Nor) after EA was decreased ( P < 0.05 vs NS group). The contents of 5-HT, 5-hydroxyindol acetic acid (5-HIAA), dopamine (DA), and homovanillic acid (HVA) in periaqueductal gray dialysate were increased (P<0.05 vs NS group). When Fen was combined with EA, the level of 5-HT and 5-HLAA were further increased (P < 0.05 vs NS + EA group). There was no obvious change of Nor, DA, and HVA. CONCLUSION: Fen potentiating EAA may be related to further activation of serotoninergic system.更多还原