【摘要】 目的:研究血管内皮生长因子(VEGF)在脑缺血再灌注引起的神经损伤中的作用,方法:用免疫组织化学方法观察VEGF在SD成年大鼠脑内的表达;用大鼠大脑中动脉阻死再灌注模型,结合TTC染色和计算机图象分析测量脑梗死体积,观察VEGF抗体对缺血性脑梗死体积的影响,结果:成年大鼠脑内的神经元上有大量VEGF表达,此表达具有脑区分布的特异性;侧脑室注射VEGF抗体,脑梗塞体积为(21.6±2.7)mm~3,对照组为(16±6)mm~3(P<0.05).结论:侧脑室注射VEGF抗体加重脑缺血再灌注后脑损伤,提示神经元表达的VEGF可能对缺血的脑组织有保护作用。更多还原

【Abstract】 AIM: To clarify the role of vascular endothelial growth factor (VEGF) in neuronal damage induced by cerebral ischemia. METHODS: Expression of VEGF in adult rat brain was measured by immunohistochemistry. Transient middle cerebral artery occlusion (MCAO) model was induced by placing a nylon thread in the lumen of the internal carotid artery. The infarct volume was shown with 2,3,5-triphenyltetrazolium chloride (TTC) staining and quantitated by computer image analyzer with and without VEGF antibody treatment. RESULTS: VEGF expression was widely distributed in neuronal cells besides vascular endothelial cells, and the neuronal distribution of VEGF was specific. After intraventricular treatment with VEGF antibody (0.1 g· L-1 daily, for 7 d following the ischemia), infarct volume in the antibody treatment was increased versus vehicle-treated rats [(21.6±2.7 vs 16±6) mm3, P <0.05] respectively. CONCLUSION: Intraventricular injection of VEGF antibody increased the infarct volume after focal cerebral ischemia in rats, suggesting that expression of neuronal VEGF may be one of neuronal protective mechanisms.更多还原