【摘要】 目的　为基因型完全相同的同卵双胞胎人类动物模型的建立积累基础资料。方法　Ｃ５７ ＢＬ６Ｊ雌性小鼠与ＫＭ 雄性小鼠交配，见栓３－５ｄ 采集小鼠晚期桑椹胚和早期囊胚，应用显微手术刀直接分割法进行胚胎二等份分割，所得半胚体外培养，观察其发育情况。结果　晚期桑椹胚分割成功率是９１－３ ％ ，半胚发育率为５７－１ ％ ，早期囊胚分割成功率约为８９－２ ％ ，半胚发育率为７１－２ ％ 。结论　Ｃ５７ ＢＬ６Ｊ 小鼠与ＫＭ 小鼠的Ｆ１ 代胚胎经切割后能在体外良好发育，该资料可用于同卵双胞胎分化发育的研究及人类双胞胎动物模型的制作。更多还原

【Abstract】 Objection To develop a animal model of human monozygotic twins. Methods C 57 BL/6J strain female mice mated with KM male mice, after seeing plug 3.5 days the compacted morulae and blastocysts were collected and bisected microsurgically by using special knife, obtained half embryos were cultured and observed. Results Take rate of bisection for morurae is 91.3%, developing rate is 57.1%, Whereas the take rate of bisection is 89.2%, the developing rate is 71.2% for blastocysts. Conclusion The F 1 demi-embryos from C 57 BL/6J and KM mice can develop in vitro after embryo bisection, the data may be useful in studying development and differentiation of monozygotic twins embryos as well as in establishing a animal model of human twins.更多还原