【摘要】 目的研究加有溴化十六烷基吡啶（ｃｅｔｙｌｐｙｒｉｄｉｕｍｂｒｏｍｉｄｅ，ＣＰＢ）的痰标本的存放时间、温度及洗涤方式对结核菌分离率的影响。方法加入ＣＰＢ的痰标本置于不同温度恒温箱内以及在２５℃恒温箱不同时间后，采用二种不同的方法洗涤后接种于培养基，分别统计阳性率。结果痰标本存放于１８℃以上较可靠，与１８℃以下相比，分离率有显著差异（Ｐ＜００５），存放１～３周分离率较高，４周后分离率明显下降，二者有显著差异（Ｐ＜００５）。结论加入ＣＰＢ的痰标本的结核菌分离率与保存温度及时间有关更多还原

【Abstract】 Objective To study the impact of store temperature,time and wash method of sputum specimens contained CPB on the separate ratio of M.tuberculosis.Method The sputum specimens contained CPB were collected and stored in different temperature,time and prepared with two wash methods,to observe and compare the separate ratio of M.tuberculosis.Result The sputum samples contained CPB are steady if stored at beyond 18℃,compare with the condition under 18℃.The positive ratio exists marked difference( P <0 05).If the samples are saved for 1～3 weeks,the positive ratio is relatively higher,and saved for over 4 weeks,the postive ratio declines boviously.There exist marked difference( P <0 05)compared with one week.Conclusion The positive ratio of M.tuberculosis of the samples contained CPB is related to the sample reseving temperature and period.更多还原