【摘要】 ＲＰ－ＨＰＬＣ法对黄芪毛状根中黄芪甲苷进行定量分析，在实验条件下，黄芪甲苷标准品在 １０．６４～５３．２０μｇ范围内与峰面积呈良好的线性关系；平均加样回收率为９２．６６％；精密度试验相 对标准偏差为４．２０％和２．４０％；测得黄芪毛状根中黄芪甲苷的含量为０．１４％（干重）。更多还原

【Abstract】 An accurate HPLC method for the determination of astragaloside Ⅳ in the hairy root cultures of Astragalus membranaceus (Fisch. ) Bunge was developed. Calibration graphs was　rectilinear between 10. 64μg and 53.20μg under the experimental conditions. The recovery was 92.66% The relative standard deviations (RSDs) of measurement precision test were 4. 20% and 2. 4.0%. The astragaloside Ⅳ content measured in the haly root cultures was 0. 14% (dry weight).更多还原