【摘要】 目的：研究硒（Ｓｅ） 和维生素Ｅ（ＶＥ） 对胰岛α细胞机能的影响。方法：分别应用天然低Ｓｅ 、人工半合成低Ｓｅ 饲料及膳食低Ｓｅ 基础上注射链脲佐菌素（ＳＴＺ） 方法结合放免分析技术，检测胰岛α细胞合成与分泌胰高糖素机能。结果：天然及人工半合成低Ｓｅ 饲料引起以胰高糖素合成与分泌减退为特点的胰岛α细胞功能异常，补一定剂量的Ｓｅ或／ 和ＶＥ 可促进α细胞分泌活动，且胰高糖素的合成与分泌基本平行。低Ｓｅ 基础上ＳＴＺ 诱导的糖尿病（ ＤＭ） 大鼠，血清胰高糖素含量显著升高，单纯补Ｓｅ 或ＶＥ 不能明显降低其血清胰高糖素水平，只有联合补Ｓｅ 和ＶＥ 才表现了明显的作用，且ＤＭ 状态下，胰高糖素的合成与释放呈不平行状态。结论：Ｓｅ 和ＶＥ 两种抗氧化微量营养素具有维持胰岛α细胞正常机能，并对其病态代谢有一定的调整作用更多还原

【Abstract】 AIM:To study effect of selenium(Se) and vitamin E on secretory function of islet α cell of rats. METHODS:Using natural and semisynthetic Se-deficient diets in combination with Se-deficiency and injection of streptozotocin methods. RESULTS:The results suggested that either natural or semisynthetic Se-deficient diet significantly declined the function of islet α cell, glucagon concentration in serum as well as in islet decreased. The diets supplemented with suitable amounts of selenium and vitamin E effectivelly prevented α cell function from declining. In diabetes mellitus rats on the basic Se-deficient diet the serum glucagon concentration increased. The diets supplemented with either selenium or vitamin E alone failed to decrease the glucagon concentration in serum of the rats but supplement of both selenium and vitamin E obviously declined the glucagon concentration. CONCLUSION:The results demonstrated that islet α cell function deponds on suitable amounts of Se and vitamin E, and it is very important that Se and vitamin E protected islet α cell from further injuries in diabetes mellitus.更多还原