【摘要】 传染性脾肾坏死病毒（ISKNV）无细菌滤液通过肌肉注射、划痕浸泡、腹腔注射和口服等四种感染途径，人工感染健康鳜鱼（Sinipercachuatsi），四种途径都能引起典型的传染性脾肾坏死病毒病。通过腹腔注射感染途径，病毒滤液在25～34℃条件下，能引起健康鳜鱼发病。另外，用病毒滤液感染尼罗非鲫（Oreochromis。niloticus）、草鱼（Ctenopharyngodonidellus）、乌鳢（Ophiocephalusargus）、大口黑鲈（Micropterussalmoides）和尖吻鲈（Latescalcarifer）五种鱼，大口黑鲈能够感染成功，为ISKNV的宿主，而其它鱼不能感染成功，不是ISKNV的宿主。更多还原

【Abstract】 The infectious spleen and kidney necrosis virus (ISKNV), derived from diseasedSiniperca chuatsi, was identified that (1 ) the virus caused ISKNV disease when challenginghealthy Siniperca chuatsi by four ways: intramuscular inoculation, intraperitoneal inoculation,wounded and bath exposure and oral inoculation; (2) the virus infected healthy Siniperca chuatsiunder 25～34℃ and the optimum temperature for infection was 28～30℃; (3 ) Microptenussalmoides was susceptibility for the virus, Oreochromis niloticus, Ctenopharyngodon idellus,Ophiocephalus argus and Lates calcarifer are not the host of ISKNV.更多还原