【摘要】 报道了小麦无性系细胞在含有３ 个Ｐ 水平（０５ 、１５ 、３５ ｍ ｍ ｏｌ·Ｌ－１） 和２ 个Ｚｎ 水平（０ 、６０μｍ ｏｌ·Ｌ－１） 的ＭＳ 培养液中，细胞对Ｐ、Ｚｎ 的积累、分布特性及交互作用的研究．Ｐ 和Ｚｎ 在细胞内的积累分别随外施Ｐ、Ｚｎ 浓度的提高而提高．与缺Ｚｎ 比较，加Ｚｎ 处理能抑制培养７ｄ 细胞中Ｐ 的含量．营养液中０５ ～１５ ｍ ｍｏｌ·Ｌ－１ Ｐ 能促进细胞的含Ｚｎ 量，但高Ｐ 处理即抑制细胞的含Ｚｎ 量．小麦细胞壁固定了细胞８３９ ％ ～８８３ ％ 的Ｐ，而外施Ｐ浓度越高，则细胞壁中Ｐ 分配的比例越大． 在供Ｚｎ 条件下，细胞壁截留了细胞中一半以上的Ｚｎ（５２０ ～６０５ ％ ） ．小麦液泡中Ｐ 和Ｚｎ 的含量较少，分别为２２ ％ ～３８ ％ 和１０６ ％ ～３０ ％更多还原

【Abstract】 This paper studied the accumulation,distribution and interaction of phosphorus and zinc in the asexual cells of wheat cultured in MS solution with three levels of phosphorus(0.5,1.5,3.5 mmol·L -1 ) and two levels of zinc(0,60 μmol·L -1 ).An application of 60μmol·L -1 Zn and 0.5～1.5mmol·L -1 P increased the reproduction and the dry matter yield of wheat cells,while the supply with no Zn or high P(3.5 mmol·L -1 ) showed an inhibited effect.The accumulation of P and Zn in cells increased with increasing P and Zn supply. In comparison with Zn deficiency,an application of 60μmol·L -1 Zn reduced the P content in cells cultured for 7 days. 0.5～1.5 mmol·L -1 P enhanced Zn accumulation in cells, but decreased it under 3.5 mmol·L -1 P. As much as 83 9%～88 3% of the total P was associated with thecell walls, and the more the P supply,the greater the P content in them.The results also showed more than half of Zn (52 0～60 5%)in cells was examined in cell walls.In contrast to the cell walls,the contents of P and Zn in vacuoles were much lower,with 2 3～3 8% and 10 6～30% respectively.更多还原