【摘要】 目的 研究甘氨脱氧胆酸(GDCA)对肝细胞脂质过氧化及细胞膜流动性的影响,探讨GDCA 肝损伤机理。方法 以 GDCA 为处理因素,体外培养肝细胞。结果 终浓度为250μmol/L 时,GDCA 组培养1～4小时,肝细胞 MDA 含量显著增加(P<0.001),细胞膜流动性显著降低(P<0.02)。随着丙二醛(MDA)的增加和细胞膜流动性的降低,肝细胞释放 ALT 增加,r 分别是0.945和0.986。结论 GDCA 能引起肝细胞 MDA 增加和膜流动性降低,从而导致肝细胞损伤,从新的角度解释了 GDCA 的肝损伤机理。更多还原

【Abstract】 Objective To expiore the mechanisms of hepatocyte injury caused by glycodeoxycholicacid(GDCA)through studies on the roles of GDCA to membrane fluidity and superoxides of lipids of hepato-cyte.Methods GDCA was used to treat hepatocytes of rat cultured in vitro and changes in cell membranefluidity and malondialdelryde(MDA)were observed.Results In the presence of GDCA(final concentrationof 25μmol/L),the concentration of hepatocyte MDA of GDCA increased significantly(P<0.001)andmembrane fluidity decreased(P<0.02)after culture of 1 or 4 hours.ALT was directly realted to MDA andfluorescence polarization(P),r was 0.945 and 0.86,respectively.Conclusion GDCA can induce the in-crease os MDA and decrease of fluidity of hepatocyte membrane,and finally cause hepatocyte injury.更多还原