【摘要】 为探讨血肠道病毒核糖核酸测定对协助诊断心肌炎的价值,本文根据肠道病毒高度保守区设计合成引物序列,应用RT—PCR方法分别对120例心肌炎患者,40例正常人血标本进行肠道病毒核糖核酸检测。结果检出率分别为54％及5％,说明RT—PCR在用于临床心肌炎诊断上具有很高的特异性和敏感性,优于间接酶链免疫吸附法。更多还原

【Abstract】 In order to evaluate the blood enterovirus RNA in diagnosis of myocarditis, 120 patients suspected of myocarditis and 40 normal individuals were assayed for enterovirus RNA by reverse transcription poly-merase chain reaction(RT-PCR)according to enterovirus highly conservative region designed and synthesized primer sequence. The results showed that the positive rates were 54% and 5% respectively .which suggested that RT-PCR be a specific, sensitive method for the diagnosis of myocarditis. The method of RT-PCR prevailed over that of ELISA.更多还原