【摘要】 以西洋参（ＰａｎａｘｑｕｉｎｑｕｅｆｏｌｉｕｍＬ．）的胚为材料，在附加ＧＡ３和ＩＢＡ的培养基上获得无菌苗。分别用含有Ｒｉ质粒的发根农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｒｈｉｚｏｇｅｎｅｓ）１５８３４菌株及经过胡萝卜提取液处理后的该菌株感染无菌苗切段，在外加萘乙酸６ｍｇ／Ｌ的ＭＳ琼脂培养基培养后获得毛状根。胡萝卜处理组较未处理组转化率提高１倍，出根时间提前１０ｄ。转化根在不含激素的培养基上可快速生长，表现为典型的毛状根性状。ＰＣＲ分析证实内含５７７ｂｐｒｏｌＣ序列的ＴＬ－ＤＮＡ已整合人转化株的染色体组。更多还原

【Abstract】 The embryo of Panax quinquefolium Lwere cultivated on the MSmedium with GA3 and IBA,and aseptic plants obtainedThe plants were infected with the Agrobacterium rhizogenes 15834 strain with Ri plasmid which treated with carrot extractThey were cultured on the MSagar medium by adding NAA 6mg/L and attained the hairy rootsThe root inducing rate of the carrotextracttreated group was twice of the nontreated groupAnd time need from inoculation to rooting was shorter 10d than that of nontreated groupThe inducing roots grew rapidly on phytohormonefree MS medium and showed typical hairy root phenotypePCR analysis confirmed the integration of TLDNA including 577bp rol C sequence in the transformed plants更多还原