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超临界流体重结晶吸附法分离富集紫杉醇粗品研究
Separation and Concentration of Crude Taxol Sample Using Supercritical Fluid CO 2 Extraction and Recrystallization Adsorption
【摘要】 比较用超临界流体CO2萃取,重结晶及重结晶-吸附法对紫杉醇粗品浸膏(含Taxol约1.0%)快速分离富集进行了研究,结果表明用重结晶-吸附法1次可快速使粗品紫杉醇浓缩富集10倍左右,分析结果用HPLC定量测定
【Abstract】 Supercritical fluid CO 2 extraction (SFE),recrystallization (SFR) and SFR adsorption were used to separate and concentrate Taxol from crude Taxol sample (contained Taxol~1.0%).The optima condition was discussed.By comparing the three methods,the SFR adsorption can concentrate rapidly Taxol about ten times concentration from the crude sample by one run.The results were quantified by HPLC.
【关键词】 超临界二氧化碳;
萃取;
重结晶;
分离富集;
紫杉醇;
【Key words】 supercritical CO 2; extraction; recrystallization; separation and concentration; taxol;
【Key words】 supercritical CO 2; extraction; recrystallization; separation and concentration; taxol;
【基金】 云南省科委应用基础研究基金
- 【文献出处】 云南大学学报(自然科学版) ,JOURNAL OF YUNNAN UNIVERSITY (NATURAL SCIENCES) , 编辑部邮箱 ,1999年04期
- 【分类号】TQ460.1
- 【被引频次】6
- 【下载频次】188