【摘要】 目的　探讨老年性白内障晶状体上皮细胞凋亡的生化特征。 方法　用ＤＮＡ 片段分析及ＴＵＮＥＬ 法共检测３１例老年性白内障患者晶状体上皮细胞凋亡。 结果　ＤＮＡ 片段分析显示，老年性白内障患者晶状体上皮细胞有ＤＮＡ“梯状”图谱，而健康成年人晶状体上皮细胞未见ＤＮＡ 断裂现象；ＴＵＮＥＬ 检测发现，老年性白内障患者晶状体上皮细胞中有凋亡细胞，而健康成年人晶状体上皮细胞中无明显的凋亡细胞。 结论　老年性白内障发生与晶状体上皮细胞凋亡有关更多还原

【Abstract】 ObjectiveTo investigate the biochemical characteristics of senile cataract lens epithelial cell apoptosis.MethodsBy DNA fragmentation assay and TUNEL staining,apoptosis from 31 samples of senile cataract lens epithelial cells were examined.ResultsBy DNA fragmentation assay,the DNA “ladder” were found in senile cataract lens epithelial cells,while no DNA “ladder” were found in normal cells;TUNEL staining showed that there were apoptotic cells in senile cataract lens epithelial cells,and few apoptotic cells were found in normal cells.ConclusionSenile cataract is associated with lens epithelial cells apoptosis.更多还原