【摘要】 目的:研究丁公藤碱（Erycibele Alkaloid）对人眼睫状肌细胞内Ca²⁺运动的影响。方法:应用双波长荧光分光光度计检测细胞内[Ca²⁺]_i浓度,研究M受体拮抗剂对丁公藤碱引起的细胞内[Ca²⁺]_i升高的影响。结果:丁公藤碱无荧光干扰作用。平均细胞内静息[Ca²⁺]_i为79.3±28.7nmol/Lol/Lol/L（n=24）。丁公藤碱引起双相的[Ca²⁺]_i升高。非选择性M受体拮抗剂Atropine（lnmol/L）,可完全阻断1μmol/L丁公藤碱引起的[Ca²⁺]_i升高;M₁受体拮抗剂Pirenzipine在低浓度（0.03nmol/L）时不能阻断1μmol/L丁公藤碱引起[Ca²⁺]_i升高,而在高浓度（1μmol/L）时可完全阻断1μmol/L丁公藤碱引起的[Ca²⁺]_i升高;M₃受体拮抗剂4-diphenyl acetoxy N-methy piperidine methiodide 在0.03μmol/L浓度时能够完全阻断（1μmol/L）丁公藤碱引起的[Ca²⁺]_i升高。结论:丁公藤碱是通过细胞膜上M₃受体触发人眼睫状肌细胞内Ca²⁺的升高。眼科学报1999;15:212—214。更多还原

【Abstract】 Purpose : To study the effect of erycibele alkaloid on cultured human ciliary muscle intracellular Ca2 + movement.Methods: Intracellular Ca2 + was studied by Fura-2 probe and muscarinic antangonist was applied.Results: Erycibele alkaloid had no any possible disturbulance on the examination system. The average resting[Ca2+ ]; was 79. 3 + 28. 7 nmol/Lol/L (n = 24). Erycibele alkaloid caused biphase [ Ca2 + ] i elevation. Non-selective muscarinic antagonist Atropine (1nmol/L) could completely block the lu,mol/L erycibele alkaloid induced [Ca2+]i elevation ; M1 antagonist Pirenzipine could not block the 1μmol/L erycibele alkaloid induced [Ca2 + ]i elevation in low concentration (0. 03nmol/L) but in higher concentration (1nmol/L) .Ma antagonist 4-diphenyl acetoxy N-methy piperidine methiodide could completely block the 1μmol/L erycibele alkaloid induced [Ca2 + ] i elevation in low and high concentration. Conclusion: The effect of erycibele alkaloid on increasing cultured human ciliary muscle cell intracellular Ca2+ is mediated by M3 receptor subtype. Eye Science 1999; 15:212- 214.更多还原