【摘要】 目的　寻求一种检测微量肿瘤细胞的方法。方法　采用ＰＣＲＥＬＩＳＡ 法检测宫颈、尿液、腹水中脱落细胞的端粒酶活性。结果　宫颈癌患者宫颈脱落细胞中端粒酶活性表达阳性率为９５－２４ ％ ；尿路上皮性癌尿液脱落细胞者为８８－１％ ；卵巢癌性腹水中脱落细胞者为１４－３ ％ 。结论　ＰＣＲＥＬＩＳＡ 法检测宫颈、尿液中脱落细胞的端粒酶活性可作为筛查宫颈癌、尿路上皮性癌的一种简单、敏感的方法。更多还原

【Abstract】 Objective To find a sensitive and non invasive assay for the detection of exfoliated tumor cells.Methods Polymerase chain reaction enzyme linked immunosorbent assay(PCR ELISA) was employed to detect telomerase activity in cervical exfoliated cells of patients with cervical cancer and in urine cells of patients with bladder cancer and in ascites cells with ovarian cancers. Results The positive rate of telomerase activity in patients with cervix,bladder or ovarine cancer was 95 24%, 88 1% and 14 3% respectively. Conclusion Telomerase activity is an important marker.It is a simple and sensitive screening method of cervical cancer and bladder cancer to detect telomerase activity in cervical or urine exfoliated cells by PCR ELISA.更多还原