【摘要】 采用分子生物学技术 ,从基因水平上诊断此次术后爆发感染的致病菌。根据分支杆菌的 16srDNA序列 ,设计合成一对引物 ,采用聚合酶链反应技术 ,并以结核分支杆菌、龟分支杆菌龟亚种和偶然分支杆菌做对照 ,对5 3株龟分支杆菌脓肿亚种临床分离株进行PCR扩增。在此基础上 ,采用 16srDNAPCR扩增体系检测 2 5 9份临床标本。结果 5 3株龟分支杆菌脓肿亚种临床分离株均被扩增出一条特异的 5 84bpDNA带。 2 5 9份临床标本 ,PCR扩增阳性率为 62 9%。在基因水平上确认了此次引起院内术后爆发感染的致病菌为龟分支杆菌脓肿亚种更多还原

【Abstract】 Objective To study the pathogen of the infection outbreak at gene level with molecular biological technology.Methods A single pair of primers were designed,according to the sequence of mycobacterium of 16s ribosomal DNA.53 clinaial strains were amplified by polymerase chain reaction with M.tuberculosis,M.chelonei and M.fortuitum as controls.On the basis of this study,259 specimen such as leison,disease tissue and normal tissue were amplified by 16s rDNA PCR.Results 584 bpPCR product for 53 clinical strains was yielded by 16s rDNA PCR.The positive rate of 259 specimen was 62 9%.Conlusionthe PCR products showed that the pathogen of the infection outbreak was M.chelonae subsp.abscessus.at gene.level.Second.the style of 16s rDNA PCR is sensitive,which can identifiy M.chelonae subsp.abscessus.更多还原