【摘要】 本文应用染色体荧光原位杂交（ＦＩＳＨ）技术，利用人９号和１４号染色体特异探针，对深低温冻存和长期传代的黑叶猴细胞株染色体畸变进行了分析。确定在长期冻存和传代过程中，一些黑叶猴细胞在Ｎｏ１２和Ｎｏ１７染色体之间发生了易位，一条Ｎｏ１７染色体发生断裂，断裂点在１７ｑ１３，断裂片段１７ｑ１３－１７ｑｔｅｒ易位到一条Ｎｏ１２染色体长臂末端，形成一条小的中着丝粒的和一条具较长长臂的衍生染色体即ｄｅｒ（１７）和ｄｅｒ（１２）。结果表明，荧光原位杂交技术用人染色体特异探针不仅能检测出人类染色体畸变，也能有效地检测灵长类动物染色体畸变更多还原

【Abstract】 In this paper, the chromosome aberration of long-term cryopreserved and subcultured Francois′ monkey (Semnopithecus francoisi) cell line(KCB 92008) was analyzed by fluoresence in situ hybridizaton (FISH) using human 9 and 14 chromosome DNA probes After compared the hybridization pattern with the G-banding pattern on the same metaphase, a translocation between Nos 12 and 17 chromosomes was identified In some Francois′monkey cells, one of chromosome No 17 was broken into two at the breakpoint 17q13, the segment(17q13-17qter) without centromere transfered to the long arm terminal of one chromosome No 12 Thus, two derivant chromosomes der(12) and der(17) were formed, the long arm of der(12) was longer than the normal partner, while the long arm of der(17) was shorter than the normal one The result indicated that the technique of FISH using human whole chromosome probes was not only a powerful tool to detect human chromosome rearrangements, but also a useful method to study the primate chromosome aberration更多还原