【摘要】 采用ＰＣＲ－ ＲＦＬＰ技术，检测了６２ 例动脉粥样硬化性脑梗塞患者和７９ 名对照者的Ｃ６７７Ｔ 突变的基因型。结果发现， ＭＴＨＦＲ基因Ｃ６７７Ｔ 突变型等位基因（Ｖ）频率在实验组和对照组中，有显著性差异（χ２＝ ４．４１，Ｐ＜ ０．０５）；三种基因型频率在两组人群中均无显著性差异。基因型频率的相对风险分析，ＡＶ基因型比ＡＡ 基因型患脑梗塞风险高１．７６ 倍；ＶＶ 基因型比ＡＡ 基因型患脑梗塞风险高３．２５ 倍。结果表明， ＭＴＨＦＲ 基因Ｃ６７７Ｔ 突变型等位基因与动脉粥样硬化性脑梗塞有一定的关联，突变基因型增加了动脉粥样硬化脑梗塞的发病风险。更多还原

【Abstract】 In order to detect the relationship between MTHFR gene C677T polymorphism and arteriosclerotic cerebral infarction, this study examined the genotype of 62 patients with arteriosclerotic cerebral infarction and 79 control subjects by PCR-RFLP. The result showed that there was significance difference between patients and control subjects in V allele frequency of MTHFR gene C677T mutation (χ 2 =4.41, P <0.05) and there was no difference between patients and control subjects in genotype frequency of MTHFR gene C677T mutation. The relative risk for arteriosclerotic cerebral infaction of heterozygote ( AV / AA ) was 1.76 and that of homozygote ( VV / AA ) was 3.25. The study confirmed an association between mutated allele of the MTHFR gene C677T and arteriosclerotic cerebral infarction, mutated genotypes increased the risk of arteriosclerotic cerebral infarction.更多还原