节点文献
黄颡鱼染色体的C─带、Ag—NORs、荧光带和复制带显带的研究
Chromosome Studies in Pelteobagrus fulvidraco by C-banding,Ag-NORs, Fluorochrome Staining and Replication Banding
【摘要】 采用Giemas染色、C─带、Ag—NORs、荧光染色和复制带显带的技术对黄颡鱼染色体进行了研究。结果表明,黄颡鱼只有部分的染色体呈现阳性C─带,可分为三类,其中NORs区是染色最深、染色面积最大的区域,为深染居间C─带。其Ag-NORs位于m5q末端。CMA3染色显示NORs区呈现出明亮的荧光。中复制染色体上着丝粒区、端粒区和居间区浅染。发现核仁缢痕、深染居间C─带、Ag—NORs、CMA3明亮区和中复制带浅染NORs区位置基本一致,C─带阳性区和中复制带浅染区具有对应性。
【Abstract】 Metaphase chromosomes of Pelteobagrus fulvidraco were analyzed by means of Giemsa staining. C-banding, Ag-NORs, fluorochrome staining and replication banding. Only parts of chromosomesshowed C-band positive, the C-band heterochromatin was located in three regions. The NORs-bearingchromosomes had the largest and strongest C-bands. The long arms of chromosome pair 5 were the regions showing positive labeling with Ag-staining. Fluorochrome CMA3 positively stained the NORs. Inmid-period, negative replication bands were located in the centromere. the terminal and interstitial regionsof the chromosomes. The distributions of secondary constri-ctions, positive C-bands, Ag-NORs, positivefluorescence bands and negative replication bands of mid-period were coincident.
【Key words】 Pelleobagrus fulvidraco; C-banding; Ag-NORs; Fluorochrome staining; Replication banding;
- 【文献出处】 遗传 ,HEREDITAS(BEIJING) , 编辑部邮箱 ,1999年02期
- 【分类号】Q343
- 【被引频次】12
- 【下载频次】155