【摘要】 利用以大赖草基因组DNA为探针的荧光原位杂交技术对导入普通小麦的大赖草染色质进行检测，结果：（1）根尖体细胞或花粉母细胞时期均可用于检测大赖草染色质。间期核中杂交信号点数与所含大赖草染色体数目之间的对应关系依染色体显强C-带末端数不同而不同；（2）利用荧光原位杂交，从普通小麦-大赖草单体异附加系的减数分裂前植株60Co-γ射线辐射后代中检测到一批大赖草端着丝粒染色体和小麦-大赖草染色体易位等结构变异；（3）用基因组原位杂交检测导入小麦的大赖草染色质时加或不加封阻DNA对原位杂交结果无太大影响。更多还原

【Abstract】 Chromatin of Leymus racemosus transferred into wheat was detected byfluorescent in situ hybridizahon (FISW using biohnlabelled genomic DNA as a probe.Received February 24, 1998, revision received Detober 2, 1998The project was supported by National Natural Science Foundation. State Science and Technology Commissionand McKnight Foundation of USAThe results are as follows. (1) Any division stages of root-tip cells and pollen mothercells could be used to identify chromatin of L racemosus by HSH. Thecorresponding relation between yellow bright dots of hybrid signs in interphase nucleusand contalned chromosomes of L racemosus varies with the chromosome tendnals ofstrong C-band. (2) Some alien chromosome structural changes such as arm deletionand common wheat-L racemosus translocation were identified by FISH from theself-pollinated progenies of T aestivum-L racemosus monosomic addition linesirradiated with Co-γ rays shortly before meiosis. (3) Blocking DNA is unnecessaryfor the detection of L racemosus chromatin by genomic HSH.更多还原