【摘要】 报道小麦×Elymusrectisetus属间杂种BC2F236个衍生系，通过染色体原位杂交和RAPD分析，检测Elymusrectisetus染色体及其特异染色体DNA标记。原位杂交结果表明，36个衍生系中大多数系含1对异源Erectisetus染色体，少数系含3条以上Ereclisetus染色体；RAPD结果表明，有13个随机引物（OPBA-08、OPB－14OPEA-09、OPF-05、OPF－09、OPJ-05、OPK-03、OPN-12、OPP-20、OPS－12、OPT-20、OPZ-09、OPZ-11）能够在这36个衍生系中分别扩增出普通小麦所没有的Erectisetus特异的染色体DNA片段。其中Erectisetus特异染色体DNA片段OPF－05480bp、OPF－09650bp和OPZ-11350bp只能够在“1048”系统的全部8个株系中扩增出；OPN-12350bp只能够在“1057”系统的全部6个株系中扩增出；OPB-14900bp和OPM-05420bp只能够在“1034”、“1026”2个系统的全部22个株系和“1057”－5－3株系中扩增出。直接获得了3个类型不同的异附加系，省去常规通过测配和附加系两两杂交F1PMCMI细胞学鉴定来确证异附加系之间的异同。由此说明染色体原位杂交和RAPD分析有机结合是鉴定异附加系（异代换系）快速有效的方法，且方法简单、易行．更多还原

【Abstract】 Detection of Elymus rectisetus chromosome segments added to wheat and RAPDmarkers in 36 derivatives of (wheat×Elymus rectisetus) were analyzed using therandom amplified polymorphic DNA (RAPD) and gonomic in sita hybridization. Thestudy of in situ hybridization demonstrated that most of 36 derivatives contained onepair E rectisetus chromosome, a few lines contained more than three E recrisetusReceived March 10, 1998, revison received August 8, 1998The project supported by National Natural Science Foundation of China (No.39870529)USDA-ARS-FRRL, UTAH STATE UNIV., Logan, UT84322-6300, USAchromosoms. The study of RAPD demonstrated that 13 of 21 primers (all operon 10mer sequences) yield 16 products that could be assigned to 36 derivatives, three of16 markers for 8 lines of "1048" (OPF-05480bp、OPF-09650bp and OPZ-11350bp), one for6 lines of "1057" (OPN-12350bp), two for 22 lines of "1034" and "1026" (OPB-14900bpand OPJ-05 420bp) Three diffrent type alien addition lines directly were obtained in thestudy- Thus it is a fast and effective method of appraising alien addition lines.更多还原