【摘要】 [目的 ]研究阿霉素对MGc 80 3胃癌细胞凋亡的诱导作用 .[方法 ]通过细胞涂片苏木精与伊红染色法、吖啶橙荧光染色法和琼脂糖凝胶电泳法 ,观察了阿霉素诱导MGc 80 3胃癌细胞凋亡的形态变化 .[结果 ]阿霉素能使MGc 80 3胃癌细胞出现典型的凋亡 .DNA电泳呈梯形带 ,进一步证实了光学显微镜所见 .[结论 ]阿霉素可诱导MGc 80 3胃癌细胞发生细胞凋亡 .更多还原

【Abstract】 OBJECTIVE To investigate the role of Adriamycin induced apoptosis in the MGc 803 of gastric carcinoma cells. METHODS The morphologic changes of adriamycin induced apoptosis in the MGc 803 of gastric carcinoma cells were examined by the methods of hematoxylin eosin, acridine orange and fluorescence staining and DNA gel electrophoresis. RESULTS Adriamycin could induce apoptosis of MGc 803 gastric carcinoma cells, which exhibited time dependent and dose dependent patterns. A typical DNA ladder band, as a characteristic of apoptosis, was also manifested in DNA gel electrophoresis. CONCLUSION The adriamycin could induce the apoptosis in gastric carcinoma cells.更多还原