【摘要】 以往我们报道了组胺H₂受体激动剂4-甲基组胺具有抑制白血病HL-60细胞增殖的作用。在本实验中对4-甲基组胺诱导HL-60白血病细胞分化的作用进行了研究。采用体外细胞培养的方法,加入不同浓度的4-甲基组胺连续培养1—6天。以NBT还原及细胞形态判定细胞分化的程度,同时测定细胞内cAMP和[Ca²⁺]i浓度的变化,以探索4-甲基组胺对HL-60白血病细胞可能的作用机理。研究结果表明,用不同浓度的4-甲基组胺处理1-6天,HL-60白血病细胞可被诱导分化为不同成熟程度的粒细胞。当用4-甲基组胺处理HL-60细胞30分钟后,细胞内的cAMP浓度呈浓度依赖性地升高。同样,细胞内钙离子浓度也呈现浓度依赖性升高。这表明4-甲基组胺诱导HL-60白血病细胞分化是通过升高细胞内。cAMP以及[Ca²⁺]i浓度介导的。更多还原

【Abstract】 The effect of histamine H2 receptor agonist, 4-methylhistamine (4-MH), was investigated on differentiation of HL-60 leukemic cells. The results of NBT reduction and cellular morphology verified that HL-60 leukemic cells were induced toward mature granulocytes when the cells were cultured with various concentrations of 4-MH for 1-6 days. Meanwhile, the intracellular levels of cAMP and cytosolic free calcium were concentration-dependently elevated by 4-MH. It was therefore postulated that the induction of differentiation of HL-60 leukemic cells by 4-MH was mediated by elevating intracellular concentrations of cAMP and free calcium ions.更多还原