节点文献
中药中绿原酸的毛细管区带电泳分析(英文)
Analysis of Chlorogenic Acid in Traditional Chinese Medicines with Capillary Zone Electrophoresis
【摘要】 本文提出了一种测定金银花和含金银花的八种中成药中绿原酸标志物的毛细管区带电泳分析方法。所用的背景电解质为含有10mmol.L-1磷酸盐和20mmolL-1硼酸盐的pH7.00的缓冲溶液,并含有5%的乙醇以提高样品的溶解度。测定的线性范围在27.7~665μg.ml-1,相关系数r=0.9996。测定金银花和含金银花的八种中成药中的绿原酸可以在匕分钟内完成。本分析方法简单、快速、适用面广。
【Abstract】 A simple, rapid and versatile capillary zone electrophoresis method wasdeveloped for the determination of chlorogenic acid as a marker in traditional Chinese medicines.The buffer solution used was a solution consisting of 10 mmol·L(-1) phosphate and 20 mmol·L(-1) boric acid containing 5% ethanol wtth the apparent pH value adjusted to 7.00. The linear calibration rangewas 27.7~65 μg·ml(-1) (r=0.9996). The contents ofchlorogenic acid in Flos Lonicerae and eigh FlosLonicerae containing Chinese medicines wee easily determined within 15 min with the sameprocedure.
【Key words】 Capillary electrophyoresis; Flos Lonicerae; Chlorogenic acid; Traditional Chinese medicine; Quantitation;
- 【文献出处】 Journal of Chinese Pharmaceutical Sciences ,中国药学(英文版) , 编辑部邮箱 ,1999年03期
- 【分类号】R284
- 【被引频次】25
- 【下载频次】157