【摘要】 目的对比研究ＰＣＲ与培养法对解脲支原体的敏感性与特异性，为临床诊断提供可靠依据。方法取患者泌尿生殖道分泌物，用ＰＣＲ和培养法对其中的解脲支原体同时进行检测。结果检测临床标本３０８份，ＰＣＲ法的阳性率为３４．７４％，培养法的阳性率为３０．１９％。以培养结果为准，ＰＣＲ法的敏感性为９４．６２％，特异性为９１．１６％。结论ＰＣＲ方法具有敏感特异、操作简便快速等优点。但该方法对实验条件和检查技术要求较高，一般实验室的检测结果准确性低。且不能鉴别标本中解脲支原体的死活情况，不能作为判愈标准，故不能取代培养法而加以推广使用。更多还原

【Abstract】 Objective To study and compare the sentivity and peculiarity of the methods of PCR and cultivation to ureaplasma urealyticum in order to supply reliable basis for clinical diagnosis.Methods The secretions from the patients’ urogenital tract were taken and ureaplasma urealyticum was tested and determined in them with PCR and cultivation at the same time.Results Of 308 clinical samples tested and determined, the positive rate was 34.74% with PCR.The positive rate with cultivation,30.19%.Taking the result of the cultivation as a standard,the sensitivity of PCR was 94.62%;the peculiarity was 91.16%.Conclusions There come the advantages of sensitivity,peculiarity,and easy and quick operation and so on with the method of PCR.As this method needs higher laboratory conditions and test technolegy, in an ordinary labouratory the accuracy of the results tested and determined are low;the life and death condition of ureaplasma urealyticum in the samples can’t be determined;and the standard of being cured or not can not be determined. So it can not replace that of cultivation and can not be widely used.更多还原