【摘要】 用质子诱导X射线发射法测定人红细胞与Gd柠檬酸和乳酸络合物温育时，胞浆、细胞膜、膜脂和膜蛋白上Gd含量随时间的变化及阴离子通道抑制剂对Gd进入细胞的影响。结果表明，Gd络离子可以从膜上的阴离子通道摄入，但并不排除其他摄入方式。Gd进入红细胞是一双时相过程，在起始阶段有一部分Gd以快速反应与细胞膜结合，之后以慢过程释放，释放的Gd中一部分又逐渐进入细胞。Gd的络合物能促进氯离子内流，但不能促进钙离子内流。氯离子内流的增加可能与Gd的络合物在细胞膜诱导的"孔洞"结构和开启了阴离子通道相关。更多还原

【Abstract】 In order to clarify the mechanism of across-membrane transport of gadollnium complexeswith different charges, the temporal changes of gadolinium content in cytosol, membrane oferythrocytes as well as membrane lipids and membrane proteins were determined by means ofproton induced X-ray emission(PIXE) method during incubation of human erythrocyltes withNa3[ Gd(citrate),] and [ Gd(Lactic)2 ] of. The innuence of anion channel blocker(DIDs) onthe permeability of erythrocytes to gadollnium was investigated. The results show that the anion complexes of gadolinium Penetrate into the cells via anion channels, but the ways other thananion channels cannot be excluded. Gadolinium transPOrt is a process with two stages. In thefirst stage the complexes of gadolinium bind very rapidly to membrane to a large extent. Thenthey are released from the membrane and small amount Penetrates inward to the cytosol slowly.In addtion, the temPOral changes of calcium and chloride content in cytosol were determinedwhen the complexes of gadolinium enter into erythrocytes. The results reveal that the complexes of gadolinium promote chlorion influx, but not calcium. The increase in chlorion influx canbe interpreted by both the "pore formation" and the induced opening of anion channels.更多还原