【摘要】 含Ⅰ型菌毛的３ 个不同血清型（Ｏ１ 、Ｏ７８ 及Ｏ８８） 的菌株， 大容量培养后提取菌毛制备３ 种单价菌毛油乳苗。用１ 日龄雏鸡分别免疫３ 种单价菌毛油乳剂苗， 每雏免疫量为１２５ μｇ ， 隔离饲养至２ 周龄经气囊攻毒， 并评价疫苗的免疫原性。结果未免疫鸡出现８７－５ ％ ～１００ ％ 的死亡率， 免疫鸡用同源菌株攻毒后死亡率仅１２－５ ％ ， 用异源菌株攻毒出现３７－５％ ～６２－５ ％ 的死亡率。免疫鸡攻毒后未死亡者， 经扑杀观察， 可见在气囊、心包及肝脏的病变非常轻微， 且攻毒后比非免疫鸡能更有效地清除攻入气囊的大肠杆菌更多还原

【Abstract】 Three avian E.coli(O 1,O 78 ,O 88 )isolates containing type Ⅰ pili were cultivated largely for preparing the pilus vaccines.Each day old chick was vaccinated with 0 25 mL(containing 125 μg)monovalent pilus vaccine and challenged via the air sac with E.coli(O 1,O 78 ,O 88 respectively)2 weeks after the vaccination with E.coli O 1,O 78 ,and O 88 ,rspectively.Unvaccinated chickens suffered 87 5%～100% mortality,vaccinated chickens suffered 12 5% morality when challenged with homologous E.coli and 37 5%～62 5% mortality when challenged with heterologous E.coli.Vaccinated chickens had very mild gross lession in the air sacs,liver and pericardial sacs.Vaccinated chickens also elminated E.coli more efficiently than unvaccinated ones after challenge.更多还原