【摘要】 用亚ＭＩＣ（药物最低抑菌浓度）连续递增式传代培养法获得了禽大肠杆菌（血清型为Ｏ１、Ｏ２、Ｏ７８）对氟喹诺酮类药物（ＦＱｓ）—诺氟沙星（ＮＦＬＸ）、依诺沙星（ＥＮＸ）、环丙沙星（ＣＰＬＸ）稳定的高度耐药菌株（ＭＩＣ提高≥１２８倍），通过对耐药菌株和敏感菌株ＤＮＡ旋转酶的分离、提取及活性检测，并进行统计学分析比较，研究ＤＮＡ旋转酶活性改变与细菌耐药的关系，结果表明：禽大肠杆菌对氟喹诺酮类药物产生耐药的一个重要因素为其ＤＮＡ旋转酶的活性改变。更多还原

【Abstract】 Stable high level FQs resistant (FQ R)strains of avian E coli were obtained by serial passages on agar containing subinhibitory concentrations of some FQs DNA gyrases obtained from these resistant strains and their original sensitive strains were examined,compared and analyzed statistically The results suggested that the DNA gyrase might played on imporfant role in the drug resistance of bacteria更多还原