【摘要】 目的: 为监测脊髓灰质炎 (以下简称脊灰) 高危人群健康儿童中, 是否还有脊灰野病毒株循环。方法: 在曾是脊灰流行区的乡镇, 于1997～1998 年定点采集0～4 岁健康儿童粪便计538 人份, 用Hep-2C细胞分离病毒; 鉴定为脊灰病毒, 采用温度特征试验作毒力测定和RT-PCR试验型内分析。结果: 在2 年的监测中, 计分离到脊灰Ⅰ型病毒10 株, Ⅲ型病毒3株, 非脊髓灰质炎肠道病毒12 株。分离的脊灰病毒温度特征试验, 两种温度滴度差均> 5, 判为弱毒(疫苗) 株; RT-PCR型内分析亦为疫苗(Sabin) 相关株, 两试验结果吻合。结论: 在具有高免疫屏障的健康儿童中, 仍有脊灰病毒循环, 但均为疫苗相关株更多还原

【Abstract】 Objective:To find whether there is still prevalence of wild strains of poliovirus in high risk group.Methods:538 faceal specimens were sampled from healthy children 0~4 year old during the period of 1997~1998 in towns and counties which were polio epidemic area before.Virus was isolated with Hep 2c cell and was identified to be poliovirus.Sensitive test of temperature and reverse transcription polymerase chain reaction(RT PCR) were applied to measure virulence and to differentiate the intratypic differecciation.Result:10 strains of type Ⅰ,3 strains of type Ⅲ poliovirus and 12 strains of NEPV were isolated during two years monitoring activity.Viruse that isolated were identified to be vaccine related strains according to the difference of two temperature titre exceeded 5 and RT PCR intratypic analysis.Conclution:Only vaccine related strains of poliovirus is in polio high risk group.更多还原