【摘要】 目的：检验氧化损伤的牛RPE细胞是否存在p53基因表达的变化和人参皂甙Rg1、Rb1和维生素E对其的影响，以期进一步研究RPE细胞氧化损害的机理.方法：建立牛RPE细胞氧化损伤模型，原位杂交技术测定RPE细胞p53mRNA的表达，免疫组化技术测定p53蛋白表达.结果：p53蛋白表达阳性的牛RPE细胞的细胞核染成黄色，与次黄嘌吟／黄嘌吟氧化酶（HX／XO）组p53蛋白阳性的细胞数相比较，正常对照组，Rg1（0.1mg.L-1）组及VitE（10mg.L-1）组存在显著性差异（P＜0.05），而与Rb1（10mg.L-1）组无显著性差异.原位杂交法测定P53mRNA表达，牛RPE细胞P53mRNA表达阳性可在细胞浆内见到位于细胞周边部的蓝紫色絮状物，分别与HX／XO组阳性的细胞相比较，正常对照组，Rg1组及VitE组存在显著性差异（P＜0.05），而与Rb1组无显著性差异.结论：氧化损伤可导致基因P53mRNA及其蛋白在RPE细胞内的表达明显增加.人参皂甙Rg1和VitE对抗氧自由基对RPE细胞损伤的机制可能是通过清除氧自由基，影响凋亡基困p53的表达，从而有效地抑制氧自由基对RPE细胞的损伤.更多还原

【Abstract】 Objechvef To inveshgate whether changes of p53 expression occur in the Oxidized RPE cells and whether ginsenoside Rb1.Rg1 and vitamin E have an influence on p53 gene expression of oxidized RPE cells. Methods: The esperimental model of oxidized bovine RPE cells was made. In these cells, mRNA expression of gene p53 was evaluated by using in situ hybridhation, the level of protein of p53 by using immunocytochendsny Results: The protein and mRNA of p53 gene were increased in RPE cells with HX/XO treatment (P<0.05). Rg1(0.1mg.L-1) and vixen E(10 mg.L-1) significantly decreases the level of protein and mRNA of p53 gene in oxidized RPE cells in comparison with HX/XO treatment(p<0.05), Rb1(10 mg.L-1) had no effect (P<0.05). Conclusionst Oxygen free radicals could change the expression of p53 gene. Ginsenoside Rg1 and vitamin E could inhibit the expression of gene p53 and Rb1 had no effect on it.更多还原