【摘要】 甲状旁腺素(parathyroid hormone,PTH)不仅在调节钙磷代谢中可促进骨发生破骨细胞性溶骨,也可促进骨的合成代谢作用。近年发现PTH还可促进成骨细胞的增殖分化。其细胞生物学和分子生物学机理尚待研究。本实验以成骨样细胞ROS17/2.8为研究材料,以胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)为细胞增殖的阳性对照,检测了PTH对DNA合成、细胞周期进程及cyclin E和cyclinA的表达,以求探讨PTH促成骨细胞增殖时对细胞周期的影响。结果表明,PTH可促进DNA合成,改变细胞周期各时相细胞比例及增加cyclin E和cyclin A的表达。该结果提示PTH可加速成骨细胞增殖周期的进程。更多还原

【Abstract】 Parathyroid hormone (PTH)is usually regarded as calcitropic hormone. Recently its non-classic function has been demonstrted that PTH plays a role in bone anabolism. The purpose of this study was to inestigate the effect of PTH on celi cycle progression in osteoblast-like ROS17/2. 8 celis,using IGF-1 as a positive control. It showed that in ROS17/ 2. 8 celis either PTH(10-9mol/L)or IGF-1 (10-9mol/L)treatment can enhance ’H-TdR incorporation 105. 1% and 120. 1% respectively,increase the percentage of S stage and G2+M stage cells,as well as increase the expression of G1 cyclin (cyclin E)and G2 cyclin (cyclin A). It suggested that PTH can accelerate the cell cycle progression in ostesblastlike ROS17/2. 8 cells.更多还原