【摘要】 从本研究建立的Ｌｅｙｍｕｓｒａｃｅｍｏｓｕｓ（Ｌａｍ．）Ｔｚｖｅｌｅｖ（大赖草）和Ｐｓａｔｈｙ－ｒｏｓｔａｃｈｙｓｊｕｎｃｅａ（Ｆｉｓｃｈ）Ｎｅｖｓｋｉ（新麦草）的ＤＮＡ文库中分别挑出５５０和３４８个重组克隆提取质粒ＤＮＡ，用普通小麦、Ｌ．ｒａｃｅｍｏｓｕｓ和Ｐｓａ．ｊｕｎｃｅａ等物种的总ＤＮＡ作探针，通过两轮点渍杂交，发现有１５个克隆稳定地与Ｌ．ｒａｃｅｍｏｓｕｓ和（或）Ｐｓａ．ｊｕｎｃｅａ有强杂交信号，与中国春无或基本无杂交信号，初步认定这些克隆为物种专化ＤＮＡ重复序列克隆。其中５个又被选取作交互杂交以测定彼此间的同源性，结果表明，ｐＰｊ６０５与ｐＰｊ２０５有同源性，而ｐＬｒ３４４，ｐＬｒ４２６和ｐＬｒ６４７相互间以及与ｐＰｊ２０５和ｐＰｊ６０５之间均无序列同源性。更多还原

【Abstract】 and 348 recombinant clones were picked out from L.racemosus and Psa.juncea libraries and plasmid DNA was isolated from each of them.The dot blots of recombinant plasmid DNA were hybridized with the32P labelled total DNA of Triticum aestivum,L.racemosus,Psa.juncea and other 4 species of Triticea.15 recombinant plasmids gave strong hybridization signals with DNA of L.racemosus and Psa.juncea but no or almost no hybridization signals with that of T.aestivum,which indicated that they could contain speciesspecific DNA repeats.Five of them were chosen for further characterization.pLr344,pLr426 and pLr647 were cloned from L.racemosus while pPj205 and pPj605 from Psa.juncea.Crosshybridization of the 5 DNA repeats indicated that pPj605 showed homology to pPj205.However,pLr344,pLr426 and pLr647 did not show homology,if any,to others.更多还原