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萆廣中薯蓣皂甙的分离及其对鸡痛风的作用

Isolation of the dioscin from rhizoma dioscoreae colletii and its effect on gout

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【作者】 童德文耿果霞赵宝玉陈正能刘恩军

【Author】 TONG De wen,GEN Guo xia,ZHAO Bao yu,CHEN Zheng neng,LIU En jun (College of Animal Science and Veterinary Medicine,Northwestern Agricultural University,Yangling,Shaanxi 712100,China)

【机构】 西北农业大学动物科学与动物医学学院!陕西杨陵712100

【摘要】 从萆廣中分离出一种白色针状结晶,经TLC和MP鉴定为薯蓣皂甙。萆薢粉经乙醇提取后制成水剂,比色法测定薯蓣皂甙质量浓度为4.785 7 g·L- 1.将30 只痛风鸡随机分成3 组,每组10 只,第Ⅰ组为对照;第Ⅱ组每日用肾肿清按10 g·kg- 1的比例拌料饲喂;第Ⅲ组每日饮水中添加52.5 m L皂甙水剂。每间隔3 d 颈静脉采血3~5 m L/只,分别测定血液尿酸、血清钙、血清磷的含量。结果表明,第Ⅱ,Ⅲ组血液尿酸含量比第Ⅰ组明显降低,差异显著(P< 0.05);第Ⅱ,Ⅲ组血清钙和血清磷含量与第Ⅰ组差异不显著(P> 0.05);第Ⅱ组血液尿酸、血清钙和血清磷含量与第Ⅲ组差异不显著(P> 0.05)。第Ⅱ组和第Ⅲ组治愈率分别为60% 和80% .

【Abstract】 Dioscin,a white needle crystal isolated from Rhizoma Dioscoreae colletii,was identified by TLC and MP.The powder of Rhizoma Dioscoreae colletii was extracted with 800 mL/L ethanol to prepare water agent of dioscin with the content of dioscin being 4.785 7 g·L -1 .30 chicks were divided into 3 groups randomly,10 chicks in each group.Group Ⅰ was control,Group Ⅱ was fed with forage containing 1 percent Shenzhongqing each day and Group Ⅲ added 52.5 mL prepared water agent in drink water each day.Blood samples (3~5 mL/chick) were collected from jugular vein with an interval of 3 days.The contents of uric acid,serum calcium and phosphorus were determined.The results revealed that the contents of uric acid in groups Ⅱ and Ⅲ were significantly lower than Group Ⅰ ( P <0.05),but there was no significant difference between groups Ⅱ and Ⅲ ( P >0.05).The contents of serum calcium and phosphorus were noticed in Group Ⅱ.There was no significant difference among three groups ( P > 0.05) .The cure rates of group Ⅱ and Ⅲ were 60 percent and 80 percent,respectively.

【关键词】 萆廣鸡痛风尿酸薯蓣皂甙
【Key words】 rhizoma dioscoreae colletiichicken gouturic aciddioscin
【基金】 西北农业大学青年基金
  • 【文献出处】 西北农业大学学报 ,THE JOURNAL OF NORTHWEST AGRICULTURAL UNIVERSITY , 编辑部邮箱 ,1999年06期
  • 【分类号】S858.313.73
  • 【被引频次】15
  • 【下载频次】102
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