【摘要】 研究了葡萄糖氧化酶（ＧＯＤ）、β－Ｄ－葡萄糖（β－Ｄ－Ｇ）、辣根过氧化物酶（ＨＲＰ）、色原物（ＣＳ）及ｐＨ 值等因素对放大系统的影响。结果表明，放大系统的最佳ｐＨ 值为６．４，理想色原物是联大茴香胺，３，３′，５，５′－四甲基联苯胺（ＴＭＢ）和邻苯二胺（ＯＰＤ）不宜作为放大系统的色原物。ＧＯＤ，β－Ｄ－Ｇ，ＨＲＰ和ＣＳ在不同程度上均极显著（Ｐ＜ ０．０１）地影响试验结果，其主次关系为ＧＯＤ＞ β－Ｄ－Ｇ＞ ＞ ＨＲＰ＞ ＣＳ．反应时间对试验结果有显著（Ｐ＜ ０．０５）影响。适当增加反应物浓度和延长反应时间对放大系统是有利的更多还原

【Abstract】 Influences of glucose oxidase (GOD),β D glucose (β D G),horseradish peroxidase (HRP),chromogeneous substance (CS) and pH value on SAS were studied in this paper.The results show that the o ptimum pH value for SAS is 6.4 and odianisidine (ODA) as CS fits the SAS of ARIS,but 3,3′,5,5′ tetramethylbenzidine and o phenylene diamine not.GOD,β D G,HRP and CS have significant effects on the SAS on different degrees respectively ( P <0.01). Their important order is GOD>β D G>>ODA>HRP.Reaction time also has significant influence on the SAS ( P <0.05).Increasing reactant concentration and prolonging reaction time benefit the SAS.更多还原