【摘要】 用含体积分数０．５％ 血清的ＤＭ ＥＭ 直接饥饿胎儿及皮肤成纤维细胞，细胞数先是略有增长，然后逐渐下降。胎儿和皮肤成纤维细胞在血清梯度饥饿期间，更换含体积分数为４％ ，２％ 和１％ 血清的ＤＭＥＭ，细胞仍缓慢增长；更换含体积分数为０．５％ 血清的培养液后，细胞数逐渐降低。血清饥饿成纤维细胞后，细胞直径明显小于对数生长期的细胞（２０，１９ＶＳ３６ μｍ ，Ｐ＜ ０．０１）。血清饥饿的成纤维细胞，ＰＣＮＡ 单抗染色阴性，证明离开了细胞周期，进入Ｇ０ 期，该期的细胞仍保持着活力更多还原

【Abstract】 After using DMEM including 0.5% NCS for direct starvation,the numbers of fetal and skin fibroblasts first increased slightly,then gradually decreased. During the serum gradient starvation,with changing DMEM including 4%,2% and 1% NCS,the numbers of fetal and skin fibroblasts still increased a little;and after being changed medium including 0.5% NCS,the numbers gradually decreased.Diameter of the fibroblast was obviously smaller than that of cells during log phase (20 μm,19 μm VS 36 μm, P <0.01) after serum starvation. Stained by antiproliferating cell nuclear antigen (PCNA),the cells after serum starvation were negative.This confirmed the cells had exited from cell cycle and been induced to G 0 phase.The cells in G 0 phase still maintained viable.更多还原