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法氏囊细胞培养上清的免疫活性

The Immunoactivity of Chicken Bursa of Fabricius Cell Culture Supernatants

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【作者】 靳亚平王爱华王春学陈祖云

【Author】 JIN Ya-ping; WANG Ai-hua; WANG Chun-xue; CHEN Zu-yun (Northwestern Agricultural University, Yangling Shaanxi 712100)

【机构】 西北农业大学动物科学与动物医学学院!陕西杨陵712100

【摘要】 法氏囊细胞培养上清经浓缩、离心后,分别进行溶血空斑形成试验和鸡NDⅣ系免疫试验。结果表明.法民囊细胞培养上清能够显著提高SRBC免疫,小鼠脾细胞的PFC数目(P<0.01).对雏鸡NDⅣ系免疫有促进作用.与免疫对照组相比.HI抗体水平平均提高1个滴度,证明体外培养的法葡萄氏囊细胞能分泌免疫活性因子。

【Abstract】 Culture supernatants of chicken or embryo bursa of fabricius were harvested, and the ef-fects of which on mouse PFC and the chicken ND Ⅳ vaccination were tested. It results that the super-natants’in vitro enhanced the index of PFC significantly (P <0. 001) in a dose-dependent manner, andincreased the titer of the antibody to ND Ⅳ vaccine in chicken serum as well- It indicates that the cells ofchicken or embryo bursa of fabricius are able to synthesize and creatc the Bursin, which has the immunoac-tivity similar to the Bursin from bursa of fabricius.

【关键词】 法氏囊溶血空斑试验免疫ND
【Key words】 Bursa of FabriciusPFCND vaccinationCulture supernatant
  • 【文献出处】 西北农业学报 ,ACTA AGRICULTURAE BOREALI-OCCIDENTALIS SINICA , 编辑部邮箱 ,1999年04期
  • 【分类号】Q932.O6
  • 【被引频次】11
  • 【下载频次】102
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