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法氏囊细胞培养上清的免疫活性
The Immunoactivity of Chicken Bursa of Fabricius Cell Culture Supernatants
【摘要】 法氏囊细胞培养上清经浓缩、离心后,分别进行溶血空斑形成试验和鸡NDⅣ系免疫试验。结果表明.法民囊细胞培养上清能够显著提高SRBC免疫,小鼠脾细胞的PFC数目(P<0.01).对雏鸡NDⅣ系免疫有促进作用.与免疫对照组相比.HI抗体水平平均提高1个滴度,证明体外培养的法葡萄氏囊细胞能分泌免疫活性因子。
【Abstract】 Culture supernatants of chicken or embryo bursa of fabricius were harvested, and the ef-fects of which on mouse PFC and the chicken ND Ⅳ vaccination were tested. It results that the super-natants’in vitro enhanced the index of PFC significantly (P <0. 001) in a dose-dependent manner, andincreased the titer of the antibody to ND Ⅳ vaccine in chicken serum as well- It indicates that the cells ofchicken or embryo bursa of fabricius are able to synthesize and creatc the Bursin, which has the immunoac-tivity similar to the Bursin from bursa of fabricius.
【关键词】 法氏囊;
溶血空斑试验;
免疫;
ND;
【Key words】 Bursa of Fabricius; PFC; ND vaccination; Culture supernatant;
【Key words】 Bursa of Fabricius; PFC; ND vaccination; Culture supernatant;
- 【文献出处】 西北农业学报 ,ACTA AGRICULTURAE BOREALI-OCCIDENTALIS SINICA , 编辑部邮箱 ,1999年04期
- 【分类号】Q932.O6
- 【被引频次】11
- 【下载频次】102