【摘要】 以全胚胎培养模型结合免疫组化和电镜技术等探讨了乙酰水杨酸和叶酸对大鼠卵黄囊及胚胎的影响。结果表明：培养基含乙酰水杨酸３００μｇ／ｍｌ组的卵黄囊直径和胚胎体长均显著低于阴性对照组，胚胎畸形率明显增高。组织学检查发现卵黄囊厚度变薄。间质层血岛、微血管数量明显减少，血管壁细胞不连续，血管发育不良。卵黄囊内皮层细胞顶端微绒毛少见。内皮层细胞的溶酶体、内质网及核蛋白体等结构和数量均有明显改变。而６０ｍｇ／ｋｇ叶酸灌胃鼠血清与乙酰水杨酸联合应用后，则卵黄囊的上述毒性作用明显减轻或消失，提示叶酸能明显拮抗乙酰水杨酸致大鼠卵黄囊的毒性作用。更多还原

【Abstract】 The antagonistic effects of folic acid to aspirin on the development of rat yolk sac and embryos were studied during early organogenesis by using whole embryo culture technique and electron microscopy.The results showed that when treated with aspirin,the diameter of yolk sac and the body length of embryo were much shorter, the frequencies of abnormal embryos were significantly higher, and the yolk sac became thinner than those without treatment. The number of blood islands and capillaries decreased, the differentiation and development of blood vessel were retarded in the treated group.Ultrastructure observation also showed that the numbers of microvilli and capillaries were greatly decreased,and the structure and the numbers of lysosomes,endoplasmic reticula and ribosomes significantly changed in the treated group.When aspirin was added with high folic acid serum,the toxicity on yolk sac decreased or disappeared. The results suggested that the protection of yolk sac would be related to the antagonism of folic acid to aspirin.更多还原