【摘要】 ＰＣＲ技术在环境微生物的检测方面已得到越来越广泛的应用，Ｓｔｅｆａｎ等［１］利用ＰＣＲ技术检测土壤中的转基因细菌，后来用于检测土壤中不可培养的微生物［２］，跟踪环境中的特定细菌或ＤＮＡ［３］，揭示土壤生态系统的基因多样性［４］等。利用ＰＣＲ技术来检测...更多还原

【Abstract】 PCR and molecular hybridization provide new methods to detect microorganisms in environment. A simple, sensitive protocol was developed to detect Bacillus thuringiensis HD 1 in soil by PCR and Southern Blotting in this study. Methods of extracting DNA from soil and purification were compared. By a modified total DNA extraction from soil and purification, followed by PCR and Southern Blotting, strain HD 1 released into soil can be detected with a sensitivity of 2 2×10 2 cells/g soil. By using other specific primers, this detection protocol can be widely used in the research of ecology and risk assessment of genetically engineered microorganisms released into environment.更多还原