【摘要】 香蕉束顶病（ＢＢＴ）是一种发生在蕉类作物的严重病害。从带有典型香蕉束顶病症状的香蕉植株中按照检测植原体的方法提取ＤＮＡ，扩增患病植株中植原体１６ＳｒＤＮＡ片段，证明香蕉束顶病中有植原体存在。对此扩增片段进行限制性酶切片段长度多态性（ＲＦＬＰ）分析和核酸序列分析，并与已知植原体的序列进行同源性比较，构建进化树。结果显示该片段与Ｇｒ１的亲缘关系最近。更多还原

【Abstract】 Extract DNA from banana plants with banana bunchy top di sease and amplify the phytoplasma 16S rDNA fragment by nested PCR. The classification of the phytoplasma established on the basis of 16S rDNA restriction patterns was examined by performing a sequence analysis of 16S rDNA fragment. The RFLP pattern, sequence data and phylogenic tree showed that this phytoplasma should belong to Group Ⅰ( Phytoplasma asteri) .更多还原