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杀鞘翅目苏云金芽孢杆菌新菌株及其杀虫剂的研究
NEW COLEOPTERANCIDAI STRAINS OF BACILIUS THURINGIENSIS AND PRODUCTION OF COLEOPTERANCIDE
【摘要】 从中国土壤中分离出2 株杀鞘翅目昆虫的苏云金芽孢杆菌( Bacillus thuringiensis)YM03 及SHQ1110 。YM03 的血清型为H8a8b ,SHQ1110 的H 血清型未知。二菌株皆产近菱形的薄扁伴孢晶体,分别含68 ~70kD 和65kD 的晶体蛋白质。毒力生物测定证明对柳蓝叶甲( Plagiodera versicolora) 及马铃薯甲虫( Leptinotarsa decemlineata) 有高毒效。发酵性能良好。YM03 粉剂田间防治马铃薯甲虫有高效。稀释400 倍喷雾,防治效果达946 % 。
【Abstract】 Two coleopterancidal strains of Bacillus thuringiensis , YM 03 and SHQ11 10, were isolated from soil in China. H Serotype of YM 03 was H8a8b,and H Serotype of SHQ11 10 has not been identified. Both of them produced flat bipyramid like crystals which consisted of 68~70kD and 65kD protien respectively. Bioassay indicated that these two Bt strains were very active on the larva of Leptinotarsa decemlineata and Plagioder aversicolora . They grew prosperously in fermentation. Powder of YM 03 product was high effect against colorado potato beetle in the field experiment.
- 【文献出处】 微生物学报 ,ACTA MICROBIOLOGICA SINICA , 编辑部邮箱 ,1999年06期
- 【分类号】S476.11
- 【被引频次】27
- 【下载频次】217