【摘要】 采用数值分类方法对分离自鸡眼草（ Ｋｕ ｍ ｍｅｒｏｗｉａ） 和木蓝（Ｉｎｄｉｇｏｆｅｒａ） 的根瘤菌及已知参比菌株进行聚类分析，发现在８３ ％ 的相似性水平上形成２ 个不同与已知菌种的新类群。以ＳＤＳ全细胞蛋白电泳技术快速聚类分群扩大菌株数，在８６ ％ 的相似性水平上，分离自鸡眼草的２４ 株菌形成第１ 类群，分离自木蓝的２０ 株菌形成第２ 类群。ＤＮＡ 同源性测定结果表明，这２ 个类群中心菌株ＳＨ７１３ 和ＳＨＬ０４２ 与１３ 个已知根瘤菌种的ＤＮＡ 同源性均小于６１ ％ 。因此，分离自鸡眼草和木蓝的根瘤菌分别构成２ 个独立的根瘤菌新种群。更多还原

【Abstract】 Therhizobialstrainsisolated from Kum merowia and Indigofera and the known reference strains were classified by performing numerical taxonomy. New isolated strains were dividedintotwo new clusters at 83% similarity level.Based on the numericaltaxono my,additionalisolatesin each cluster were studied by using SDS PAGEof whole cell pro tein.Twenty-fourstrainsisolated from Kum merowia fellinto cluster 1 . Twenty strains isolated from Indigofera fellinto cluster2 .Theresultsof G+ Cmol% and DNAhomology analysisshowedthatthe DNAhomologiesbetweenthecentralstrain SH713 and SHL042 and the 13typestrains werelessthan 61% .Thus,therhizobialstrainsfrom Kum merowia and Indigofera weretwo new individualspecies of Rhizobium .更多还原