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产胞外木聚糖酶链霉菌发酵条件的正交试验
AN ORTHOGONAL EXPERIMNT ON FERMENTATION CONDITIONS WITH EXTRACELLULAR XYLANASE PRODUCING STRAIN OF Streptomyces sp.
【摘要】 通过正变试验,设计出适合链霉菌(Streptomycessp.Strz—6)产胞外木聚糖酶的发酵条件,培养基(g/L)含麦草半纤维素5,NaNO31.67,酵母膏2,乳糖2,Tween801.5K2HPO4·3H2O1,MgSO4·7H2O0.5,NaCl0.3,微量元素B、Mn、Fe、Zn、Mo。接种量为1.4×108个孢子/50ml培养基,振荡,(120r/min)培养5d.
【Abstract】 A suitable fermentation condition was designed for extracellular xylanase producing Streptomyces strain z - 6 through an orthogonal experiment. The medium contained 0. 5 % of wheat straw hemicellulose,0. 17% of NaNO3, 0. 2% of lactose, 0. 15% of Tween 80, 0. 1% of K2HPO4.3H2O, 0. 05% of MgSo4·7H2O, 0. 03% of NaC1, and some trace elements (B, Mn, Fe, Zn, and Mo. ). 1. 4 ×108 spores were inoculated in 50ml medium in 250ml Erlenmeyer flask in a water shaker (120rpm, 32t ) for 5d stirring.
【关键词】 胞外木聚糖酶;
链霉菌(Streptomycessp.Strz-6);
发酵;
正交试验;
【Key words】 extracellular xylanase; Streptomyces sp. str z-6; fermentation; orthogonal experiment;
【Key words】 extracellular xylanase; Streptomyces sp. str z-6; fermentation; orthogonal experiment;
【基金】 山东省科委资助
- 【文献出处】 微生物学杂志 ,JOURNAL OF MICROBIOLOGY , 编辑部邮箱 ,1999年04期
- 【分类号】TQ920.1
- 【被引频次】22
- 【下载频次】185