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利用原生质体技术选育聚β-羟基丁酸高产菌株的研究

STUDY ON THE SELECTION OF HIGH-YIELD PHB PRODUCING STRAIN BY TECHNIQUE OF PROTOPLASTS

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【作者】 薛林贵王维国姜双林

【Author】 Xue Lingui;Wang Weiguo;Jiang Shuanglin (Departwent of biology, Qingyang College, Xi Feng, 745000)

【机构】 庆阳师范高等专科学校生物系!西峰745000

【摘要】 从六份城市生活污水样品中,分离筛选出一菌株,具如下主要特征:杆菌,0.85~1.2×2.0~5.0μm;革兰氏染色阴性;具衣鞘,细胞包在鞘里,丝状体很长,但无分枝;亚端生鞭毛;胞内具PHB颗粒和异染颗粒;液化明胶微弱;菌落为光滑型和丝状两种。经鉴定该菌株为浮游球衣细菌(Sphaerotilusnatans)。该菌在28℃条件下生长良好,细菌细胞大,PHB颗粒很大,有的可以充满整个细胞。经培养的菌种于培养液中在室温下保存近一年,方法简便效果好。

【Abstract】 A Strain of sphaerotilus natans from six polluted water sampleswas isolated. The strain has some main features as followst bacillus, 0.85~1.2 ×2.0~ 5.0μm; Gramnegahve bacteria; it has sheath that cells in it,the hypha is vare long but no divaricahon; subterminal--flagellum; there are metachromatic granules and PHB granules in cells; there are two kind of colony, the one is smooth, and the other is silk The strain can grow properly Under condition 28℃ and produce big PHB granules.The strain was kept in cultivating liquid under home temperature near to a year, this is a good and handy way.

【关键词】 球衣细菌分离鉴定PHB
【Key words】 SphaerorilusisolationidenhficahonPHB.
【基金】 甘肃省教委科研基金!965-08
  • 【文献出处】 微生物学通报 ,MICROBIOLOGY , 编辑部邮箱 ,1999年03期
  • 【分类号】Q939.97
  • 【被引频次】15
  • 【下载频次】111
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