【摘要】 用聚合酶链式反应（ＰＣＲ）从ＨＩＶ１ｇａｇ基因中扩增出衣壳蛋白Ｐ２４截短体（ｔＰ２４）基因，插入质粒ｐＧＥＸ４Ｔ３中构成重组表达质粒ｐＧＥＸｔｐ２４，将ｐＧＥＸｔｐ２４转化大肠杆菌ＢＬ２１后获得了高效表达，表达量占菌体总蛋白量的３４３８％。经Ｇｌｕｔａｔｈｉｏｎｅ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析纯化的截短体Ｐ２４纯度为９２７７％。纯化的截短体Ｐ２４在间接ＥＬＩＳＡ和免疫印迹检测ＨＩＶ抗体阳性血清和正常人血清中，具有很高的抗原特异性和免疫反应性。更多还原

【Abstract】 A truncated gene partly encoding HIV 1 capsid protein (P24) was amplified by PCR from HIV 1 gag genome and inserted into a expression plasmid pGEX 4T3.The recombinant plasmid pGEX tp24 was trasfered into E.coli(BL 21 ) and expressed by IPTG induction.After being purified by Glutathione Sepharose 4B affinity chromatography,the recombinant truncated P24 showed strong antigentic specificity and immunoreactivity when reacted with HIV Ab positive sera in indirect ELISA and Western blot.更多还原