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普通Taq DNA聚合酶扩增幽门螺杆菌尿素酶基因长片段
General Taq DNA polymerase amplified long DNA fragments of Helicobacter pylori urease
【摘要】 ＰＣＲ反应扩增较长的ＤＮＡ片段比较困难。本实验对幽门螺杆菌染色体ＤＮＡ上的２７Ｋｂ尿素酶基因用普通ＴａｑＤＮＡ聚合酶进行扩增，扩增结果以琼脂糖凝胶电泳证实，得到所需的２７Ｋｂ的片段。该实验为今后幽门螺杆菌工作的进一步研究提供了经济，有效，简捷的条件。
【Abstract】 Routine PCR reaction to amplify long DNA fragments is difficult.Our research tried to amplify 2.7 kb urease gene in Helicobacter pylori chromosome DNA using routine Taq DNA polymerase.The agarose gel electrophoresis proved the results that 2.7 kb DNA fragments had been amplified successfully.This experiment provided us a economic,effcient and simple method for later research on Helicobacterpylori urease study.
- 【文献出处】 微生物学免疫学进展 ,PROGRESS IN MICROBIOLOGY AND IMMUNOLOGY , 编辑部邮箱 ,1999年02期
- 【分类号】R392-33
- 【被引频次】1
- 【下载频次】84