【摘要】 目的：研究胃癌癌旁组织中抑癌基因和抗凋亡基因蛋白的表达及其内在关系。方法：胃镜及外科手术中取４０例 胃癌患者的癌组织和癌旁组织各两块，石蜡包埋，切片ＨＥ染色作病理及免疫组织化学检查ｐ５３、ｐ１６和ｂｃｌ－２基因蛋 白表达。结果：ｐ５３在胃癌组织中阳性表达率４２．５％，癌旁组织１２．５％，有显著性差异（Ｐ＜０．０１），其表达强度也有显著 性差异（Ｐ＜０．０１）；ｐ１６在癌旁组织阳性表达率为６０％，显著高于胃癌组织３７．５％（Ｐ＜０．０５），其表达强度也有显著性差 异（Ｐ＜０．０５）；ｂｃｌ－２在胃癌组织和癌旁组织阳性表达率无显著性差异（６７．５％比４７．５％，Ｐ＞０．０５），其表达强度也无显 著性差异（ｐ＞０．０５）。ｐ５３与ｐ１６、ｐ１６与ｂｃｌ－２表达之间均无显著相关性（Ｐ＞０．０５），而ｐ５３与ｂｃｌ－２表达之间存在显著相 关性（Ｐ＜０．０５）。结论：在胃癌发生发展过程中，抑癌基因（ｐ５３、ｐ１６）４抗凋亡基因（ｂｃｌ－２）之间存在既独立又协同的作 用。更多还原

【Abstract】 Background/Aims: To investigate the expression and mutual relationship of tumor suppression gene and hc1-2 proteins in gastric carcinoma and surrounding tissues. Methods: 40 patients with gastric carcinoma underwent endoscopy and surgical resection. Samples were taken from tumor site and paracancerous tissues, and ABC immunohistochemical staining was us ed to detect the expression of p53, p16 and bc1-2 proteins. Results: The positivity rate and staining intensity of mutant p53 gene expression in gastric cancer were significantly higher than those in paracancerous tissues (P<0.01); The positivity rate and staining intensity of p16 gene expression in paracancerous tissues were significantly higher than those in gastric cancer (P<0.05). There were no significant differences for the positivity rate and staining intensity of hcf-2 gene expression in gastric cancer and in paracancerous tissues (P>0.05). There was no significant correlation not only between p53 and p16 (P>0.05), but also between p16 and hc1-2 (P>0.05), whereas there was significant correlation between p53 and hc1-2 (P<0.05). Conclusions: p53, p16 and hc1-2 play not only separate but synergetic role in the formation and development of gastric cancer.更多还原