【摘要】 ＩＬ－１β对醋氨酚（ＡＡＰ）引起小鼠在体肝脏的损伤具有保护作用，本工作旨在观察ＩＬ－１β抗肝损伤效应是直接作用于肝细胞发挥的还是通过其它途径间接发挥的，探讨ＩＬ－１β抗肝损伤作用的传递机制。方法向无血清培养２４小时的原代肝细胞加人２０ｍＭｏｌ／Ｌ的ＡＡＰ，在加醋氨酚前６０ｍｉｎ和ｓｏｗｉｎ，向不同组分别加人ＩＬ－１β和／或Ｌ－ＮＡＭＥ（Ｎ－ｎｉｔｒｏ－Ｌ－ａｒｇｉｎｉｎｅｍｅｔｈｙｌｅｓｔｅｒ）。加ＡＡＰ后继续培养一段时间，测培养液中ＡＬＴ和ＡＳＴ的活性及肝细胞内ｃＡＭＰ和ｃＧＭＰ的含量。结果ＩＬ－１β可完全抑制醋氨酚引起的肝细胞培养液中ＡＬＴ、ＡＳＴ的升高，不能逆转醋氨酚引起的肝细胞内ｃＡＭＰ含量的降低，但与正常组相比，ＩＬ－１β可提高正常肝细胞内ＣＧＭＰ的含量（Ｐ＜０．０５）和醋氨酚损伤组的肝细胞内ｃＧＭＰ的含量（Ｐ＜０．０１）。加人ＮＯ合酶抑制剂Ｌ－ＮＡＭＥ可抑制ＩＬ－１肝细胞保护作用。结论ＩＬ－１β对肝细胞有直接的保护作用，其可能是一种有效的肝保护剂。这种保护可能与ＩＬ－１β引起的肝细胞内ＮＯ、ｃＧＭＰ改变有关。更多还原

【Abstract】 Aim To observe how the protective effect of IL-1β on liver damaged by aceednophen (AAP ) take Place; Tostudy its conducting routes. Methods Acedrinophen was given in bepatocytes cultural 24 hours in serum free medium. LNAME, this was given espectively before AAP in dtherent group. The activities of ALT and AST, the contents of cAMP andcGMP were measured in each group at certain time. Results The increased leakages of ALT and AST induced by AAP wereinhibited by IL-1β. The decline of cAMP content reversed by IL-1β. But IL-1β could increase cGMP content in normal and AAPinjured hepatocytes. But this could be abolished by adrinistheion of byNAME before IL-1β given. Conclusion IL-1β can atleast partially protect against the damage of AAP on hepatocytes. cGMP may act as the second messenger for the action on thehepatocytes for IL-1β.更多还原