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D-核糖提取工艺的研究
STUDY ON THE SEPARATING PROCESS OF D RIBOSE
【摘要】 核糖发酵液用饱和醋酸铅除去菌体、杂蛋白,然后通过AB-8 树脂进行脱色,脱色液分别经过001×7,201×7 树脂脱盐后,经过浓缩,于201×4 硼酸型阴离子交换树脂进行分离。以0.02 m ol/L四硼酸钠缓冲液为洗脱液,将其中核糖流分浓缩、加甲醇除去硼酸根后,加乙醇减压浓缩得到白色核糖固体。
【Abstract】 The culture medium removed microbe and protein by adding about 10% saturated PbAc.Treated with AB 8.001×7 and 201×7 resins to decolor and desalt,then passed through 201×4 anion exchange resins (BO - 3),washed with 0.01 0.02 mol/L Sodium tetraborate to exclude Glucose,pentose solution was concentrated and Boric acid was excluded as methyl borate under reduced pressure,D ribose was crysted with addition of EtoH.
- 【文献出处】 天津轻工业学院学报 ,JOURNAL OF TIANJIN INSTITUTE OF LIGHT INDUSTRY , 编辑部邮箱 ,1999年04期
- 【分类号】Q532.2
- 【被引频次】7
- 【下载频次】233